Composite
Part:BBa_K4165203
Designed by: Mennatallah Mahmoud Mohamed Abdelzaher Turky Group: iGEM22_CU_Egypt (2022-10-01)
Trim-(GSGS)-Docs
This parts code for the trim21 E3 ligase having his PRYSPRY domain truncated (BBa_K3396007), fused to type 1 Dockerin module derived from Clostridium thermocellum cellulosome scaffoldin using Glycine serine flexible linker repeated two times to maintain part flexibility needed during target ubiquitination
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 225
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 182
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |